Volume 2, Issue 3, May 2014, Page: 27-32
Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS
Nasir Hassan Wagini, Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria
Amira Shawky Soliman, Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt
Mohamed Said Abbas, Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt
Yasser Adel Hanafy, Medical and Aromatic Plants Unit, Desert Research Center, Cairo, Egypt
El-Saady Mohamed Badawy, Medicinal and Ornamental Plant, Faculty of Agriculture, Cairo University, Cairo, Egypt
Received: Mar. 25, 2014;       Accepted: Apr. 16, 2014;       Published: May 10, 2014
DOI: 10.11648/j.plant.20140203.11      View  2829      Downloads  400
This study designed to compare phytochemicals for both Nigerian and Egyptian henna using preliminary test for secondary metabolites, thin layer chromatography (TLC), Fourier Transform Infrared spectroscopy (FTIR), and Gas Chromatography Mass spectroscopy (GCMS). Lawsonia inermis L. (Lythraceae) commonly called henna known for its cosmetic properties. The active ingredients in the leaves were extracted and fractionated using chloroform, ether, methanol, and ethyl-acetate. The resultant fractions primarily analyzed for secondary metabolites and secondarily. The phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, quinines, resins and sterols. The presence or absence of all the phytochemicals in these solvents was more or less similar between both samples. TLC profiling shows 9 bands of chemical compounds that are significantly similar in both samples. The FTIR spectrum confirmed the presence of 8 functional in both samples and an additional of aliphaticiodo compound in Egyptian sample. Sixteen and seventeen compounds identified in Nigerian and Egyptian samples respectively by GCMS analysis. Lawsone (2-hydroxy 1, 2-naphtoquinone) is one out of 7 common chemical compounds identified in both samples. The common compound identified by TLC, FTIR, and GCMS were found to be significantly similar in both quantity and quality. Thus, the research confirmed henna to be an important source of phytochemicals of immense pharmaceutical significance.
Henna, Lawsonia Inermis, Phytochemicals, Nigeria, Egypt
To cite this article
Nasir Hassan Wagini, Amira Shawky Soliman, Mohamed Said Abbas, Yasser Adel Hanafy, El-Saady Mohamed Badawy, Phytochemical Analysis of Nigerian and Egyptian Henna (Lawsonia Inermis L.) Leaves using TLC, FTIR and GCMS, Plant. Vol. 2, No. 3, 2014, pp. 27-32. doi: 10.11648/j.plant.20140203.11
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