Volume 3, Issue 1, January 2015, Page: 1-7
Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes
Esra Maltas, Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey
Aslı Dageri, Department of Plant Protection, Faculty of Agriculture, Ankara U
Hasibe Cingilli Vural, Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey
Salih Yildiz, Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey
Received: Aug. 21, 2014;       Accepted: Sep. 9, 2014;       Published: Dec. 15, 2014
DOI: 10.11648/j.plant.20150301.11      View  2358      Downloads  232
Abstract
Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.
Keywords
Echinacea Purpurea and Echinacea Pallida, Molecular Marker, PCR, nDNA Antioxidant Activity, Fatty acid, Flavonoid
To cite this article
Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz, Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes, Plant. Vol. 3, No. 1, 2015, pp. 1-7. doi: 10.11648/j.plant.20150301.11
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