Volume 4, Issue 6, November 2016, Page: 45-50
Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in Carica papaya L., cv. Red-Lady
Md. Humayun Kabir, Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Savar, Dhaka, Bangladesh
Md. Ziaur Rahman, Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan
Ahmad Nazri Karim Mamun, Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Savar, Dhaka, Bangladesh
Received: Aug. 23, 2016;       Accepted: Sep. 8, 2016;       Published: Oct. 18, 2016
DOI: 10.11648/j.plant.20160406.11      View  2446      Downloads  90
Abstract
Somatic embryogenesis creates a number of opportunities to facilitate large-scale propagation, synthetic seed production, genetic improvement through somaclonal variation, in vitro mutagenesis, protoplast fusion and genetic transformation. Induction of somatic embryogenesis from the vegetative parts of papaya plant was met with low success rates and a slow process of regeneration. Success depends on the choice of explants, the species being used and on various methods of embryogenesis will study. The most suitable explant for somatic embryogenesis is a large portion of either meristematic tissue or cell that retain an ability to express totipotency. Various investigations were made on somatic embryo induction of Carica spp. such as ovules of immature fruits, zygotic embryos from immature fruits, axillary buds, peduncles of immature fruits etc. In this study, somatic embryo induction from zygotic embryo of an immature fruit was examined. Various concentrations of 2,4-D and two levels of sucrose concentrations with or without addition of glutamine were assessed to determine the best response for induction of somatic embryogenesis from immature zygotic embryo of immature fruits in papaya cv. Red-lady (Carica papaya L.). The results showed that MS medium supplemented with 5.0 mg/l 2,4-D + 400 mg/l glutamine + 60 g/l sucrose promoted the formation of highest (70%) percentage of somatic embryo and also the average maximum number (35.80±4.40) of somatic embryo per explant. The highest (44%) percentage of somatic embryo germination into complete plantlets were obtained on MS medium devoid of plant growth regulators. The embryo developed only shoots of 22% and only roots of 10% instead of complete plantlet formation in this medium of MS0. The average highest (4.25 ± 0.32 cm) shoot length per germinated somatic embryo derived complete plantlets were also achieved on MS0 medium. The maximum (90%) percentage of root induction and average highest (7.50 ± 0.75) number of root per somatic embryo derived shoot and also the average maximum (5.20 ± 0.40 cm) root length were obtained on MS medium without plant growth regulators. The survival rate of somatic embryo derived plantlets in the field was about 10% to 50% depending on management practices.
Keywords
Somatic Embryogenesis, Regeneration, Papaya
To cite this article
Md. Humayun Kabir, Md. Ziaur Rahman, Ahmad Nazri Karim Mamun, Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in Carica papaya L., cv. Red-Lady, Plant. Vol. 4, No. 6, 2016, pp. 45-50. doi: 10.11648/j.plant.20160406.11
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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