Volume 4, Issue 6, November 2016, Page: 71-77
Effect of Nitrogen Source and Concentration on Growth and Activity of Nitrogen Assimilation Enzymes in Roots of a Moroccan Sorghum Ecotype
Reda Ben Mrid, Laboratory of Biochemistry and Molecular Genetics, Faculty of Science and Technology, Tangier, Morocco
Redouane El Omari, Laboratory of Biochemistry and Molecular Genetics, Faculty of Science and Technology, Tangier, Morocco
Mohamed Nhiri, Laboratory of Biochemistry and Molecular Genetics, Faculty of Science and Technology, Tangier, Morocco
Received: Sep. 23, 2016;       Accepted: Oct. 8, 2016;       Published: Oct. 31, 2016
DOI: 10.11648/j.plant.20160406.14      View  2722      Downloads  82
Abstract
Carbon and nitrogen metabolism pathways are regulated by complex mechanisms in order to optimize growth and development of plants. This study was conducted to contribute to the determination of roles of some key enzymes of carbon and nitrogen metabolism in sorghum roots. Sorghum were grown under nitrate (NO3-), ammonium (NH 4+) or nitrate combined to ammonium at different concentrations. Growth parameters and protein content were evaluated. In addition, we analyzed key enzymes activities involved in nitrogen and carbon metabolism such as: nitrate reductase (NR), glutamine synthetase (GS), aspartate aminotransferase (AAT) and phosphoenolpyruvate carboxylase (PEPC). At high nitrogen levels (ammonium or nitrate), the sorghum roots showed an increase in biomass accompanied by an increase in protein content. Likewise, high concentrations induced accumulation of the non-photosynthetic isoform of phosphoenolpyruvate carboxylase. Glutamine synthetase and aspartate aminotransferase activities were also increased, especially at high levels of ammonium. These results showed that sorghum plants have an efficient system for nitrogen assimilation in their roots, allowing this plant to tolerate excessive concentration of this nutrient.
Keywords
Sorghum, Nitrogen Stress, Nitrogen Metabolism, Ammonium, Nitrate
To cite this article
Reda Ben Mrid, Redouane El Omari, Mohamed Nhiri, Effect of Nitrogen Source and Concentration on Growth and Activity of Nitrogen Assimilation Enzymes in Roots of a Moroccan Sorghum Ecotype, Plant. Vol. 4, No. 6, 2016, pp. 71-77. doi: 10.11648/j.plant.20160406.14
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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