Volume 4, Issue 6, November 2016, Page: 78-90
The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars
Mohamed Ahmed Seif El-Yazal, Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
Hala Fawzi Eissa, Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt
Safia Mahmoud Abd El-Mageed Ahmed, Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
Saad Mohamed Howladar, Biology Department, Faculty of Sciences, Albaha University, Albaha, Saudi Arabia
Safi-naz Sabet Zaki, Department of Water Relations and Field Irrigation, National Research Centre, Dokki, Cairo, Egypt
Mostafa Mohamed Rady, Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt
Received: Sep. 17, 2016;       Accepted: Oct. 12, 2016;       Published: Oct. 31, 2016
DOI: 10.11648/j.plant.20160406.15      View  2816      Downloads  94
The mtlD gene-contained transgenic wheat has established the role of mannitol and sugars accumulation in alleviating the abiotic stresses, including salinity. This study was conducted to determine whether the 85 mM NaCl-salinity could be tolerated by wheat (genotype 235/3) plants of which seeds were transformed with mtlD gene (from Escherichia coli). The effects of mtlD gene transformation into wheat seeds on growth traits, physio-biochemical attributes, and yield and its quality of transgenic wheat genotype were investigated compared to non-transgenic wheat genotype under 85 mM NaCl-salinity. Results showed that mtlD gene-contained transgenic plants had improved salt tolerance over non-transgenics, showing by better growth traits (i.e., number of leaves and leaf area per plant, root system size and plant dry weights), physio-biochemical attributes (i.e., levels of leaf chlorophylls, shoot free proline, total soluble sugars, soluble sugar fractions and mannitol, activities of enzymatic and non-enzymatic antioxidants, and contents of nutrient elements), yield (i.e., number of spikes and grain weight per plant, and 1000-grain weight) and yield quality (i.e., grain contents of starch, protein and soluble sugars). The mtlD gene transformation into wheat seeds appears to a better strategy to increase salt tolerance of plants through increased performance of mannitol and sugar accumulation, showing more of their salt stress-protecting role. The best performing mtlD transgenics could be incorporated in a breeding program to accumulate transgenes for stress tolerance in elite wheat genotypes in a step to commercialize these transgenics with the proper level of salt tolerance.
Physio-Biochemical Attributes, Salt Stress, Transgenic Wheat, Yield and Its Quality
To cite this article
Mohamed Ahmed Seif El-Yazal, Hala Fawzi Eissa, Safia Mahmoud Abd El-Mageed Ahmed, Saad Mohamed Howladar, Safi-naz Sabet Zaki, Mostafa Mohamed Rady, The mtlD Gene-overexpressed Transgenic Wheat Tolerates Salt Stress Through Accumulation of Mannitol and Sugars, Plant. Vol. 4, No. 6, 2016, pp. 78-90. doi: 10.11648/j.plant.20160406.15
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