Volume 5, Issue 3, May 2017, Page: 51-60
Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott)
Asseng Charles Carnot, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Ebongo Lobe Emmanuel, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Nanda Djomou Giresse Ledoux, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Akono Ntonga Patrick, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Mbida Jean Arthur, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Ngono Ngane Annie, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Ambang Zachée, Faculty of Science, Department of Plant Biology, Laboratory of Phytopathology and Microbiology, University of Yaounde, Yaounde, Cameroon
Monkam Tchamaha Fabrice, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Djouokep Léonel Gautier, Faculty of Science, Department of Plant Biology, Laboratory of Plant Biology and Physiology, University of Douala, Douala, Cameroon
Received: Jul. 14, 2017;       Accepted: Jul. 21, 2017;       Published: Aug. 22, 2017
DOI: 10.11648/j.plant.20170503.12      View  1486      Downloads  55
Abstract
The cultivation of taro is of great economic and social importance on a global scale. The current orientation towards agricultural production and the serious consequences of the mildew of taro to crops prompts to find alternatives to chemical control. This study aims at selecting in the taro habitat (leaves, rhizosphere) the microorganisms with high antagonistic potential capable of ensuring the biological control of P. colocasiae. Fungus isolated from the taro-infected leaves of the cultivar "Macumba or Ibo coco" from the V8-Agar medium, was kept in pure culture. The different antagonists were obtained by two trapping techniques using P. colocasiae as bait for the associated microorganisms and by the decimal dilution technique. The results reveal fourteen antagonist isolates, including five fungi and two bacteria isolated from the leaves; Against 4 bacteria and 3 fungi at ground level. Identification of the latter identified the presence of Penicillium Sp, Trichoderma Sp, Aspergillus Sp, Pythium Sp., Bacillus Sp, Rhizobium, Streptomyces and seven other unidentified isolates (Ni). The different in vitro tests showed that Rhizobium and Ni4 showed the strongest inhibitions (91.66 and 90.69%). The greenhouse tests showed the high-inhibitory effect of Trichoderma Sp. and Rhizobium, which showed very low foliar alteration percentages (9.65 and 1.86%). These antagonists would be of particular benefit to farmers in the development of biological pesticides.
Keywords
Taro Mildew, Phytophtora colocasiae, Biological Control, Antagonist Microorganisms
To cite this article
Asseng Charles Carnot, Ebongo Lobe Emmanuel, Nanda Djomou Giresse Ledoux, Akono Ntonga Patrick, Mbida Jean Arthur, Ngono Ngane Annie, Ambang Zachée, Monkam Tchamaha Fabrice, Djouokep Léonel Gautier, Study of Antagonistic Beneficial Microorganisms to Phytophtora colocasiae, Causal Agent of Taro Mildew (Colocasia esculenta (L.) Schott), Plant. Vol. 5, No. 3, 2017, pp. 51-60. doi: 10.11648/j.plant.20170503.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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