Volume 6, Issue 1, March 2018, Page: 16-23
Seedling Resistance to Stem Rust (Puccinia graminis f.sp.tritici) and Molecular Marker Analysis of Resistance Genes in Some Wheat Cultivars
Tesfaye Letta, Oromia Agricultural Research Institute, Addis Ababa, Ethiopia
Received: Mar. 22, 2018;       Accepted: Apr. 12, 2018;       Published: May 16, 2018
DOI: 10.11648/j.plant.20180601.13      View  1008      Downloads  58
Stem rust caused by Puccinia graminis Pers.f.sp.tritici Eriks.and E.Henn.(Pgt) is one of the most destructive diseases of wheat which causing considerable yield losses in wheat growing areas worldwide. It has become a renewed threat to global wheat production after the emergence and spread of race TTKSK (also known as Ug99) and related races from Africa. Races of the pathogen in the “Ug99 lineages” are of international concern due to their virulence for widely used stem rust resistance genes and their spread throughout Africa. Disease resistant cultivars provide one of the best means for controlling stem rust. Bale zone, located on the Southeast part of Ethiopia, is one of the main wheat growing regions, playing a pivotal role in the wheat stem rust epidemic in Ethiopia. This study investigated levels of resistance in key wheat cultivars (lines) grown in Bale zone using seedling resistance evaluation and marker aided selection. Twenty wheat cultivars were evaluated for their response to stem rust infection at seedling stage under green house condition. Wheat cultivars were challenged with four stem rust races viz TTKSK, TRTTF, TTTTF and JRQCQ. A high level of phenotypic variation was observed in response to these races in the test entries, allowing for selection in these germplasm as a pre-breeding work. Out of the tested cultivars, three wheat cultivars exhibited low infection types (0–2) response to all the four races and hence selected as a source of resistance to stem rust. In addition, the existence of Sr2, Sr22, Sr24, Sr25, Sr26, Sr35 and Sr36 genes in wheat cultivars were assessed using specific DNA markers. Using molecular markers, resistance gene Sr2 was identified in 2 cultivars and Sr24 in five cultivars. However, no Sr25, Sr26, Sr35 and Sr36 were identified in any cultivars tested using DNA markers. The results of both seedling evaluation and marker based resistance gene identification will enable to breed wheat varieties with durable resistance to stem rust disease.
Cultivars, DNA Markers, Puccinia Graminis, Seedling Resistance
To cite this article
Tesfaye Letta, Seedling Resistance to Stem Rust (Puccinia graminis f.sp.tritici) and Molecular Marker Analysis of Resistance Genes in Some Wheat Cultivars, Plant. Vol. 6, No. 1, 2018, pp. 16-23. doi: 10.11648/j.plant.20180601.13
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Babiker E, Ibrahim A, Yen Y, Stein J (2009) Identification of amicrosatellite marker associated with stem rust resistancegene Sr35 in wheat. Aust J Crop Sci 3:195–200.
Beteselassie, N., C. Fininsa, and A. Badebo. 2007. Sources of resistance to stem rust (Pucciniagraminis f. sp. tritici) in Ethiopian tetraploid wheat accessions. Genet. Resour. Crop Evol. 54:337–343. doi:10.1007/s10722-005-5561-6.
Botma V, Liezel H, Robert FP, Haydar K, Cornelia MB, Zacharias AP. 2011. Characterization of two new Pucciniagraminisf. sp. triticiraces within the Ug99 lineage in South Africa. Euphytica, 179: 119±127.
Dundas IS, Anugrahwati DR, Verlin DC, Park RF, Bariana HS, Mago R, Islam AKMR. 2007. New sources of rustresistance from alien species: meliorating linked defectsand discovery. Aust J Agric Res 58:545–549.
FAO. Available: http://www.fao.org/agriculture/crops/rust/stem/stem-pathotypetracker/ Stemeffectivesrgenes/ en/. Accessed 7 Feb 2018
Gyarfas J. 1978. Transference of disease resistance fromTriticumtimopheevii to Triticumaestivum. Master’s thesis. University of Sydney, Australia.
Haile JK, Hammer K, Badebo A, Nachit MN, Roder MS. 2013. Genetic diversity assessment of Ethiopian tetraploidwheat landraces and improved durum wheat varieties using microsatellites and markers linkedwith stem rust resistance. Genetic Resources and Crop Evolution. 2013; 60: 513±527.
Hare RA, McIntosh RA. 1979. Genetic and cytogenetic studiesof durable, adult-plant resistances in Hope and relatedcultivars to rusts. Z Planzen 83:350–367.
Jin Y, Singh RP, Ward RW, Wanyera R, Kinyua M, Njau P, Pretorius ZA et al. 2007. Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Pucciniagraminis f. sp. tritici. Plant Dis. 91: 1096-1099.
Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward RW, Fetch TJ. 2008. Detection of virulence to resistance gene Sr24 within race TTKS of Pucciniagraminisf. sp. tritici. Plant Dis. 2008; 92: 923±926.
Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward R, Fetch T Jr et al . 2008. Detection of virulence to resistance gene Sr24 within race TTKS of Pucciniagraminis f. sp. tritici. Plant Dis. 92: 923-926.
Khan R, Bariana H, Dholakia B, Naik S, Lagu M, Rathjen A, Bhavani S, Gupta V. 2005. Molecular mapping of stemand leaf rust resistance in wheat. TheorAppl Genet111:846–50.
Klindworth DL, Miller JD, Jin Y, Xu SS. 2007. Chromosomal locations of genes for stem rust resistance in monogenic lines derived from tetraploid wheat accession ST464. Crop Sci. 47: 1441-1450.
Kokhmetova AM and Atishova MN. 2012. Identification of sources of resistance to wheat stem using molecularmarkers. Russ J Genet Appl Res. 2012; 2: 486±493.
Maccaferri, M., M. C. Sanguineti, P. Mantovani, A. Demontis, A. Massi, K. Ammar, J. Kolmer, J. Czembor, S. Ezrati, and R. Tuberosa. 2010. Association mapping of leaf rust response in durum wheat. Mol. Breed. 26:189–228. doi:10.1007/s11032-009-9353-0.
Mago R, Bariana HS, Dundas IS, Spielmeyer W, Lawrence GJ, Pryor AJ, Ellis JG. 2005. Development of PCR markersfor the selection of wheat stem rust resistance genes Sr24and Sr26 in diverse wheat germplasm. TheorAppl Genet111:496–504.
McIntosh RA, and Wellings CR, Park RF. 1995. Wheat rusts, anatlas of resistance genes. CSIRO, Melbourne.
Miranda LM, Perugini L, Srnic´ G, Brown-Guedira G, MarshallD, Leath S, Murphy JP. 2007 Genetic mapping of aTriticummonococcum derived powdery mildew resistancegene in common wheat. Crop Sci 47:2323–2329.
Nazari K, Mafi M, Yahyaoui A, Singh RP, Park RF. 2009. Detection of wheat stem rust race (Pucciniagraminisf. sp. tritici) TTKSK (Ug99) in Iran. Plant Dis. 2009; 93: 317.
Olivera PD, Jin Y, Rouse, Badebo A, Fetch T, Jr., Singh RP, and Yahyaoui AM et al. 2012. Races of Pucciniagraminis f. sp. tritici with combined virulence to Sr13 and Sr9e in a field stem rust screening nursery in Ethiopia. Plant Dis. 96: 623-628.
Pardey PG, Beddow JM, Kriticos DJ, Hurley TM, Park RF, Duveiller E, et al. 2013. Right-sizing stem-rustresearch. Science. 2013; 340: 147±148. doi: 10.1126/science.122970 PMID: 23580514.
Patpour M, Hovmøller MS, Justesen AF, Newcomb M, Olivera P, Jin Y, et al. 2016. Emergence of virulence to SrTmpin the Ug99 race group of wheat stem rust, Pucciniagraminisf. sp. tritici, in Africa. Plant Dis. 2016; 2: 552.
Pretorius ZA, Singh RP, Wagoire WW, Payne TS. 2000. Detection of virulence to wheat stem rust resistance gene Sr31 in Pucciniagraminisf. sp. triticiin Uganda. Plant Dis. 2000; 84: 203.
Roelfs AP, Martens JW. 1988. An international system of nomenclature for Pucciniagraminis f. sp. tritici. Phytopathol. 78: 526-533.
Roelf AP, Long DL, Robert JJ. 1993. Races of Pucciniagraminis in the United States during 1990. Plant Dis. 77: 125-128.
Singh RP, Hodson DP, Jin Y, Huerta-Espino J, Kinyua MG, Wanyera R, et al. 2006. Current status, likelymigrations and strategies to mitigate the threat to wheat production from raceUg99 (TTKS) of stemrust pathogen. CAB Rev Perspectives Agric Veterinary SciNutr Nat Resour. 2006; 1:13.
Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Peter N, Ruth W, et al. 2008. Will stem rust destroy theworld's wheat crop? Advances in Agronomy. 2008; 98: 271±309.
Singh RP, Huerta-Espino J, Bhavani S, Singh D, Singh PK, Herrera-Foessel SA, Njau P, Wanyera R, Jin Y. 2009. Breeding for minor gene-based resistance to stem rust ofwheat. Proceedings of Borlaug Global Rust Initiative, C. D. Obregon.
Spielmeyer W, Sharp PJ, Lagudah ES. 2003. Identification andvalidation of markers linked to broad-spectrum stem rustresistance gene Sr2 in wheat (Triticumaestivum L.). CropSci 43:333–336.
Stakman EC, Steward DM, Loegering WQ. 1962. Identification of physiologic races of Pucciniagraminis var. tritici. U S Dep. Agric. Agric. Res. Serv. E-617.
Stokstad E. 2007. Deadly wheat fungus threatens world's breadbaskets. Science. 2007; 315: 1786±1787. doi: 10.1126/science.315.5820.1786 PMID: 17395806.
Umesh G, Sarvjeet K, Rakesh Y, Neha S, Kajal T, Aakash KG. 2015. Recent trends and perspectives ofmolecular markers against fungal diseases in wheat. Front Mic. 2015 Aug 25. doi: 10.3389/fmicb.2015.00861 PMID: 26379639.
Vanzetti L. S., Campos P., Demichelis M., Lombardo L. A., Aurelia P. A., Vaschetto L. M., et al. (2011). Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers. Electronic J. Biotechnol. 14 1-6.
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